ASPERGILLUS FUMIGATUS annotation definitions -------------------------------------------- 2-4-2007 Annotations were compiled by Daniela Albrecht and Franziska Lessing. They were checked for compliance with MIAPE Gel-Electrophoresis V.1.2. but go beyond this standard. heading1no heading1 heading2no heading2 heading3no heading3 1 2D_gel_common_annotatios 1 General_features 1 - 1 - 1 2D_gel_common_annotatios 1 General_features 2 - 2 - 1 2D_gel_common_annotatios 1 General_features 3 - 3 - 1 2D_gel_common_annotatios 1 General_features 4 - 4 - 1 2D_gel_common_annotatios 1 General_features 5 - 5 - 1 2D_gel_common_annotatios 1 General_features 6 - 6 - 1 2D_gel_common_annotatios 2 Sample 7 - 7 - 1 2D_gel_common_annotatios 2 Sample 8 - 8 - 1 2D_gel_common_annotatios 2 Sample 9 - 9 - 1 2D_gel_common_annotatios 2 Sample 10 - 10 - 1 2D_gel_common_annotatios 2 Sample 11 Loading_buffer 11 - 1 2D_gel_common_annotatios 2 Sample 11 Loading_buffer 12 - 1 2D_gel_common_annotatios 2 Sample 11 Loading_buffer 13 - 1 2D_gel_common_annotatios 3 Gel 12 First_dimension_(IEF) 14 - 1 2D_gel_common_annotatios 3 Gel 12 First_dimension_(IEF) 15 - 1 2D_gel_common_annotatios 3 Gel 12 First_dimension_(IEF) 16 - 1 2D_gel_common_annotatios 3 Gel 12 First_dimension_(IEF) 17 - 1 2D_gel_common_annotatios 3 Gel 12 First_dimension_(IEF) 18 - 1 2D_gel_common_annotatios 3 Gel 12 First_dimension_(IEF) 19 - 1 2D_gel_common_annotatios 3 Gel 12 First_dimension_(IEF) 20 Acrylamide_concentration 1 2D_gel_common_annotatios 3 Gel 12 First_dimension_(IEF) 21 - 1 2D_gel_common_annotatios 3 Gel 12 First_dimension_(IEF) 22 Acrylamide:Crosslinker_ratio 1 2D_gel_common_annotatios 3 Gel 12 First_dimension_(IEF) 23 - 1 2D_gel_common_annotatios 3 Gel 12 First_dimension_(IEF) 24 - 1 2D_gel_common_annotatios 3 Gel 12 First_dimension_(IEF) 25 Electrophoresis_protocol 1 2D_gel_common_annotatios 3 Gel 13 Inter-dimension_process 26 - 1 2D_gel_common_annotatios 3 Gel 13 Inter-dimension_process 27 - 1 2D_gel_common_annotatios 3 Gel 13 Inter-dimension_process 28 - 1 2D_gel_common_annotatios 3 Gel 13 Inter-dimension_process 29 - 1 2D_gel_common_annotatios 3 Gel 14 Second_dimension_(SDS_PAGE) 30 - 1 2D_gel_common_annotatios 3 Gel 14 Second_dimension_(SDS_PAGE) 31 - 1 2D_gel_common_annotatios 3 Gel 14 Second_dimension_(SDS_PAGE) 32 - 1 2D_gel_common_annotatios 3 Gel 14 Second_dimension_(SDS_PAGE) 33 - 1 2D_gel_common_annotatios 3 Gel 14 Second_dimension_(SDS_PAGE) 34 - 1 2D_gel_common_annotatios 3 Gel 14 Second_dimension_(SDS_PAGE) 35 Physical_dimensions 1 2D_gel_common_annotatios 3 Gel 14 Second_dimension_(SDS_PAGE) 36 - 1 2D_gel_common_annotatios 3 Gel 14 Second_dimension_(SDS_PAGE) 37 Acrylamide_concentration 1 2D_gel_common_annotatios 3 Gel 14 Second_dimension_(SDS_PAGE) 38 - 1 2D_gel_common_annotatios 3 Gel 14 Second_dimension_(SDS_PAGE) 39 Acrylamide:Crosslinker_ratio 1 2D_gel_common_annotatios 3 Gel 14 Second_dimension_(SDS_PAGE) 40 - 1 2D_gel_common_annotatios 3 Gel 14 Second_dimension_(SDS_PAGE) 41 - 1 2D_gel_common_annotatios 3 Gel 14 Second_dimension_(SDS_PAGE) 42 - 1 2D_gel_common_annotatios 4 Protein_Finding 15 Detection 43 - 1 2D_gel_common_annotatios 4 Protein_Finding 15 Detection 44 - 1 2D_gel_common_annotatios 4 Protein_Finding 15 Detection 45 - 1 2D_gel_common_annotatios 4 Protein_Finding 15 Detection 46 - 1 2D_gel_common_annotatios 4 Protein_Finding 15 Detection 47 - 1 2D_gel_common_annotatios 4 Protein_Finding 15 Detection 48 - 1 2D_gel_common_annotatios 4 Protein_Finding 15 Detection 49 - 1 2D_gel_common_annotatios 4 Protein_Finding 15 Detection 50 - 1 2D_gel_common_annotatios 4 Protein_Finding 15 Detection 51 - 1 2D_gel_common_annotatios 4 Protein_Finding 16 Image_acquisition 52 - 1 2D_gel_common_annotatios 4 Protein_Finding 16 Image_acquisition 53 - 1 2D_gel_common_annotatios 4 Protein_Finding 16 Image_acquisition 54 - 1 2D_gel_common_annotatios 4 Protein_Finding 16 Image_acquisition 55 - 1 2D_gel_common_annotatios 4 Protein_Finding 16 Image_acquisition 56 - 1 2D_gel_common_annotatios 4 Protein_Finding 16 Image_acquisition 57 - 1 2D_gel_common_annotatios 4 Protein_Finding 17 Image 58 - 1 2D_gel_common_annotatios 4 Protein_Finding 17 Image 59 - 1 2D_gel_common_annotatios 4 Protein_Finding 17 Image 60 Dimensions 1 2D_gel_common_annotatios 4 Protein_Finding 17 Image 61 - 1 2D_gel_common_annotatios 4 Protein_Finding 17 Image 62 - 1 2D_gel_common_annotatios 4 Protein_Finding 17 Image 63 - 1 2D_gel_common_annotatios 4 Protein_Finding 17 Image 64 - 1 2D_gel_common_annotatios 5 Post_gel_annotations 18 Protein_identification 65 - 1 2D_gel_common_annotatios 5 Post_gel_annotations 18 Protein_identification 66 - 1 2D_gel_common_annotatios 5 Post_gel_annotations 18 Protein_identification 67 - 1 2D_gel_common_annotatios 5 Post_gel_annotations 18 Protein_identification 68 - 1 2D_gel_common_annotatios 5 Post_gel_annotations 19 Data_preprocessing 69 - 1 2D_gel_common_annotatios 5 Post_gel_annotations 19 Data_preprocessing 70 Normalisation 1 2D_gel_common_annotatios 5 Post_gel_annotations 19 Data_preprocessing 71 Filtering 2 Experiment_specific_annotations 6 sample_preparation 20 - 72 - 2 Experiment_specific_annotations 6 sample_preparation 21 - 73 - 2 Experiment_specific_annotations 6 sample_preparation 22 - 74 - 2 Experiment_specific_annotations 6 sample_preparation 23 - 75 - 2 Experiment_specific_annotations 6 sample_preparation 24 - 76 - 2 Experiment_specific_annotations 7 Pre-culture 25 - 77 - 2 Experiment_specific_annotations 7 Pre-culture 26 - 78 - 2 Experiment_specific_annotations 7 Pre-culture 27 - 79 - 2 Experiment_specific_annotations 7 Pre-culture 28 - 80 - 2 Experiment_specific_annotations 7 Pre-culture 29 - 81 - 2 Experiment_specific_annotations 7 Pre-culture 30 - 82 - 2 Experiment_specific_annotations 7 Pre-culture 31 C_source 83 - 2 Experiment_specific_annotations 7 Pre-culture 31 C_source 84 - 2 Experiment_specific_annotations 7 Pre-culture 31 C_source 85 - 2 Experiment_specific_annotations 7 Pre-culture 31 C_source 86 - 2 Experiment_specific_annotations 7 Pre-culture 31 C_source 87 - 2 Experiment_specific_annotations 7 Pre-culture 31 C_source 88 - 2 Experiment_specific_annotations 8 Main-culture 32 - 89 - 2 Experiment_specific_annotations 8 Main-culture 33 - 90 - 2 Experiment_specific_annotations 8 Main-culture 34 - 91 - 2 Experiment_specific_annotations 8 Main-culture 35 - 92 - 2 Experiment_specific_annotations 8 Main-culture 36 - 93 - 2 Experiment_specific_annotations 8 Main-culture 37 C_source 94 - 2 Experiment_specific_annotations 8 Main-culture 37 C_source 95 - 2 Experiment_specific_annotations 8 Main-culture 37 C_source 96 - 2 Experiment_specific_annotations 8 Main-culture 37 C_source 97 - 2 Experiment_specific_annotations 8 Main-culture 37 C_source 98 - 2 Experiment_specific_annotations 8 Main-culture 37 C_source 99 - 2 Experiment_specific_annotations 8 Main-culture 38 Temporary_additive 100 - 2 Experiment_specific_annotations 8 Main-culture 38 Temporary_additive 101 - 2 Experiment_specific_annotations 8 Main-culture 39 Growth_condition_shift 102 - 2 Experiment_specific_annotations 8 Main-culture 39 Growth_condition_shift 103 - 2 Experiment_specific_annotations 8 Main-culture 39 Growth_condition_shift 104 - 2 Experiment_specific_annotations 8 Main-culture 39 Growth_condition_shift 105 - 2 Experiment_specific_annotations 8 Main-culture 39 Growth_condition_shift 106 - lastheadingno ano nextano annotation vno nextvno value 1 19001 19002 Date_of_entry_day 0 0 [] 2 19002 19003 Date_of_entry_month 0 0 [] 3 19003 19004 Date_of_entry_year 0 0 [] 4 19004 19005 Responsible_person 19001 19002 Olaf_Kniemeyer 4 19004 19005 Responsible_person 19002 19003 Franziska_Lessing 4 19004 19005 Responsible_person 19003 19004 Andre_Schmidt 4 19004 19005 Responsible_person 19004 19005 Martin_Voedisch 4 19004 19005 Responsible_person 19005 19006 Silke_Steinbach 5 19005 19006 Institutional_adress_and_email 19006 19007 Hans_Knoell_Institute_(HKI),_Beutenbergstrasse_11a,_07745_Jena,_mailto:firstname.surname(at)hki-jena.de 6 19006 19007 Electrophoresis_type 19007 19008 Difference_gel_electrophoresis_(2D_DIGE) 6 19006 19007 Electrophoresis_type 19008 19009 Two-dimensional_(2D_standard) 7 19007 19008 Organism 19009 19010 Aspergillus_fumigatus 8 19008 19009 Strain 19010 19011 Af293 8 19008 19009 Strain 19011 19012 WT_ATCC_46645 8 19008 19009 Strain 19012 19013 ATCCDyap1 8 19008 19009 Strain 19013 19014 ATCCDskn7 8 19008 19009 Strain 19014 19015 ATCCDyap1/Dskn7 8 19008 19009 Strain 19015 19016 ATCC(CEA17)PksP 8 19008 19009 Strain 19016 19017 ATCCKu80Daif 8 19008 19009 Strain 19017 19018 pooled_standard 9 19009 19010 Type 19018 19019 control 9 19009 19010 Type 19019 19020 test 9 19009 19010 Type 19020 19021 standard 10 19010 19011 Label 19021 19022 coomassie 10 19010 19011 Label 19022 19023 silver 10 19010 19011 Label 19023 19024 Cy2 10 19010 19011 Label 19024 19025 Cy3 10 19010 19011 Label 19025 19026 Cy5 10 19010 19011 Label 19026 19027 Sypro_Ruby 11 19011 19012 Components 19027 19028 bromphenole_blue_(comassie) 11 19011 19012 Components 19028 19029 lysis_buffer_3+40mM_DTT+3.2%Servalyt_(DIGE) 12 19012 19013 Volume 19029 19030 10_mikrol 12 19012 19013 Volume 19030 19031 4_x_sample 13 19013 19014 Amount_of_protein 0 0 [microg] 14 19014 19015 Description 19031 19032 IPG_strip_native 14 19014 19015 Description 19032 19033 IPG_strip_gradient 15 19015 19016 Model_name 19033 19034 Immobiline_Drystrip_(GE_Healthcare) 16 19016 19017 Model_number_(enter_NA_if_unknown) 0 0 [] 17 19017 19018 Batch_number_(enter_NA_if_unknown) 0 0 [] 18 19018 19019 Length 0 0 [cm] 19 19019 19020 pH_range_and_distribution 19034 19035 3-7_linear 19 19019 19020 pH_range_and_distribution 19035 19036 3-7_nonlinear 19 19019 19020 pH_range_and_distribution 19036 19037 3-10_linear 19 19019 19020 pH_range_and_distribution 19037 19038 3-10_nonlinear 19 19019 19020 pH_range_and_distribution 19038 19039 4-7_linear 19 19019 19020 pH_range_and_distribution 19039 19040 7-10_nonlinear 20 19020 19021 Type 19040 19041 fixed 20 19020 19021 Type 19041 19042 linear 20 19020 19021 Type 19042 19043 stepped 20 19021 19022 Miminum 0 0 [%] 20 19022 19023 Maximum 0 0 [%] 21 19023 19024 Sample_application 19043 19044 rehydration-loading 21 19023 19024 Sample_application 19044 19045 cup-loading_anode 21 19023 19024 Sample_application 19045 19046 cup-loading_catode 21 19023 19024 Sample_application 19046 19047 cup_loading_middle 22 19024 19025 Name_of_crosslinker 19047 19048 bis-acrylamide 22 19025 19026 Ratio_(e.g._0.5)_(enter_NA_if_unknown) 0 0 [] 23 19026 19027 Additional_substances_in_gel 19048 19049 none_(0M) 24 19027 19028 Number_of_Gel_lanes_and_Sample_loading_per_lane 19049 19050 not_applicable 25 19028 19029 Running_buffer 19050 19051 none 25 19029 19030 Additional_buffers 19051 19052 rehydration_buffer_3_(7M_urea,_2M_thiourea,_2%_Chaps,_1%_Zwittergent,_bromphenol_blue) 25 19030 19031 Conditions 19052 19053 7cm_protocol_(hold-200V-10min_hold-500V-10min_gradient-to-3500V-1.5h_hold-3500V-1.5h,_room_temperature) 25 19030 19031 Conditions 19053 19054 24cm_protocol_(gradient-to-300V-4h_gradient-to-600V-3(4)h_gradient-to_1000V-4h_gradient-to-8000V-4h,_hold8000V-3h,_room_temperature) 26 19031 19032 Name 19054 19055 equilibration 27 19032 19033 Buffer 19055 19056 equilibration_buffer_(in_200ml_72g_urea,_69ml_glycerol,_4g_SDS,_10ml_tris-hcl_buffer_pH_8.8+1%DTT_(fist_step)_or_+2.5%_iodacetamid_(second_step)) 28 19033 19034 Additional_reagents 19056 19057 none 29 19034 19035 Special_equipment_and_protocols 19057 19058 none 30 19035 19036 Description 19058 19059 SDS_gel_native 30 19035 19036 Description 19059 19060 SDS_gel_gradient 31 19036 19037 Manufacturer 19060 19061 HKI_(12.5%_gele,_125ml_acrylamid-bisacrylamid,_75ml_1.5M_trisbuffer_ph_8.8,_68ml_water,_25ml_Rhinohyde,_3ml_10%_SDS,_75microl_TEMED,_3.7ml_10%_APS) 31 19036 19037 Manufacturer 19061 19062 HKI_(ready_to_use_Biometra_kit_(Laemmli_Chemie)) 32 19037 19038 Model_name 19062 19063 HKI_self_made_native 32 19037 19038 Model_name 19063 19064 HKI_self_made_gradient 33 19038 19039 Model_number_(enter_NA_if_unknown) 0 0 [] 34 19039 19040 Batch_number_(enter_NA_if_unknown) 0 0 [] 35 19040 19041 Width_(distance_from_anode_to_catode) 0 0 [cm] 35 19041 19042 Length 0 0 [cm] 35 19042 19043 Depth 0 0 [cm] 36 19043 19044 Molecular_weight_range_and_distribution 19064 19065 8-150kDa_logarithmic_apparent 36 19043 19044 Molecular_weight_range_and_distribution 19065 19066 8-200kDa_logarithmic_apparent 36 19043 19044 Molecular_weight_range_and_distribution 19066 19067 20-150kDa_logarithmic_apparent 36 19043 19044 Molecular_weight_range_and_distribution 19067 19068 20-200kDa_logarithmic_apparent 37 19044 19045 Type 19068 19069 fixed 37 19044 19045 Type 19069 19070 stepped 37 19044 19045 Type 19070 19071 linear 37 19045 19046 Miminum 0 0 [%] 37 19046 19047 Maximum 0 0 [%] 38 19047 19048 Sample_application 19071 19072 standard_procedure_combining_strip_and_gel 39 19048 19049 Name_of_crosslinker 19072 19073 bis-acrylamide 39 19049 19050 Ratio_(e.g._0.5)_(enter_NA_if_unknown) 0 0 [] 40 19050 19051 Additional_substances_in_gel 19073 19074 none_(0M) 41 19051 19052 Number_of_Gel_lanes_and_Sample_loading_per_lane 19074 19075 not_applicable 42 19052 19053 Running_buffer 19075 19076 10_x_SDS_running_buffer_(in_1l_water_30g_tris,_144g_glycin,_10g_SDS,_upper_chamber_2_x_SDS,_lower_chamber_1_x_SDS) 42 19053 19054 Additional_buffers 19076 19077 none 42 19054 19055 Conditions 19077 19078 1W/gel-1h_15W/gel-3.5h,_20_degC 42 19054 19055 Conditions 19078 19079 5W/gel-0.5h_17W/gel-3.5h,_20_degC 43 19055 19056 Direct_detection_process 19079 19080 visible_staining 43 19055 19056 Direct_detection_process 19080 19081 fluorescent_staining 44 19056 19057 Manufacturer_of_detection_agents 19081 19082 Roth 44 19056 19057 Manufacturer_of_detection_agents 19082 19083 GE_Healthcare 45 19057 19058 Detection_agent 19083 19084 coomassie 45 19057 19058 Detection_agent 19084 19085 silver 45 19057 19058 Detection_agent 19085 19086 Cy2 45 19057 19058 Detection_agent 19086 19087 Cy3 45 19057 19058 Detection_agent 19087 19088 Cy5 45 19057 19058 Detection_agent 19088 19089 Sypro_Ruby 46 19058 19059 Volume_(enter_NA_if_unknown) 0 0 [microl] 47 19059 19060 Concentration_(enter_NA_if_unknown) 0 0 [mM] 48 19060 19061 Additional_reagents_and_buffers 19089 19090 none 49 19061 19062 Special_equipment_and_protocols 19090 19091 none 50 19062 19063 Protocol 19091 19092 scanning 51 19063 19064 Indirect_detection 19092 19093 none 52 19064 19065 Type_of_equipment 19093 19094 laser_scanner 52 19064 19065 Type_of_equipment 19094 19095 light_scanner 53 19065 19066 Name_of_equipment 19095 19096 Typhoon_9200 54 19066 19067 Software 19096 19097 ImageScanner_II_(Amersham_Biosciences) 54 19066 19067 Software 19097 19098 Labscan_(comes_together_with_Image_Master_2D_Platinum) 54 19066 19067 Software 19098 19099 Scan_Control_(comes_together_with_Typhoon_9200) 55 19067 19068 Calibration 19099 19100 automatic 55 19067 19068 Calibration 19100 19101 manual 56 19068 19069 Protocol 19101 19102 scanning_gel_matrix_of_second_dimension 57 19069 19070 Imaging_software 19102 19103 DeCyder_6.5_(GE_Healthcare) 57 19069 19070 Imaging_software 19103 19104 ImageMaster_2D_Platinum_5.0_(GE_Healthcare) 57 19069 19070 Imaging_software 19104 19105 Delta2D_3.4_(Decodon) 58 19070 19071 ID 0 0 [] 59 19071 19072 Format 19105 19106 tiff 60 19072 19073 length_(pH_direction) 0 0 [pixels] 60 19073 19074 width_(MW_direction) 0 0 [pixels] 61 19074 19075 Resolution_(enter_NA_if_unknown) 0 0 [microm/pixel] 62 19075 19076 Bit-depth_(enter_NA_if_unknown) 0 0 [] 63 19076 19077 Location 19106 19107 local_server_at_HKI_(T) 63 19076 19077 Location 19107 19108 local_users_pc_at_HKI 64 19077 19113 Orientation 19108 19158 standard_(highest_MW_top,_highest_pH_right) 65 19113 19114 Spot_picking 19158 19159 manual 65 19113 19114 Spot_picking 19159 19160 automatic_(Proteineer_sp) 66 19114 19115 Digestion 19160 19161 manual 66 19114 19115 Digestion 19161 19162 automatic_(Proteineer_dg) 67 19115 19116 Ms_device 19162 19163 ESI_ion_trap 67 19115 19116 Ms_device 19163 19164 ESI_triple_quadrupole_spectrometers 67 19115 19116 Ms_device 19164 19165 ESI_sector_field_spectrometer 67 19115 19116 Ms_device 19165 19166 Bruker_Ultraflex_1_MALDI_TOF/TOF_spectrometer 68 19116 19117 Software 19166 19167 Mascot_Proteinscape 69 19117 19118 background_subtraction 19167 19168 none 69 19117 19118 background_subtraction 19168 19169 automatically_(DeCyder) 69 19117 19118 background_subtraction 19169 19170 automatically_(Image_Master_Platinum) 69 19117 19118 background_subtraction 19170 19171 automatically_(Delta2D) 70 19118 19119 First_normalisation 19171 19172 none 70 19118 19119 First_normalisation 19172 19173 logarithmising 70 19118 19119 First_normalisation 19173 19174 internal_standard_(division_by_Cy2,_DIGE) 70 19118 19119 First_normalisation 19174 19175 variance_stabilisation_(DIGE) 70 19118 19119 First_normalisation 19175 19176 loess_regression 70 19119 19120 Second_normalisation 19176 19177 none 70 19119 19120 Second_normalisation 19177 19178 logarithmising 70 19119 19120 Second_normalisation 19178 19179 internal_standard_(division_by_Cy2) 70 19119 19120 Second_normalisation 19179 19180 variance-stabilisation 70 19119 19120 Second_normalisation 19180 19181 loess_regression 70 19120 19121 Third_normalisation 19181 19182 none 70 19120 19121 Third_normalisation 19182 19183 logarithmising 70 19120 19121 Third_normalisation 19183 19184 internal_standard_(division_by_Cy2) 70 19120 19121 Third_normalisation 19184 19185 variance-stabilisation 70 19120 19121 Third_normalisation 19185 19186 loess_regression 70 19121 19122 Fourth_normalisation 19186 19187 none 70 19121 19122 Fourth_normalisation 19187 19188 logarithmising 70 19121 19122 Fourth_normalisation 19188 19189 internal_standard_(division_by_Cy2) 70 19121 19122 Fourth_normalisation 19189 19190 variance-stabilisation 70 19121 19122 Fourth_normalisation 19190 19191 loess_regression 71 19122 19123 First_filter 19191 19192 none 71 19122 19123 First_filter 19192 19193 Fold_Change 71 19122 19123 First_filter 19193 19194 Z_Score 71 19123 19124 Threshold_first_filter 0 0 [] 71 19124 19125 Second_filter 19194 19195 none 71 19124 19125 Second_filter 19195 19196 t-test_p-value 71 19124 19125 Second_filter 19196 19197 ANOVA_p-value 71 19124 19125 Second_filter 19197 19198 t-test_p-value_corrected_for_multiple_comparisons 71 19124 19125 Second_filter 19198 19109 ANOVA_p-value_corrected_for_multiple_comparisons 71 19125 19078 Threshold_second_filter 0 0 [] 72 19078 19079 Experimental_context 19109 19110 Carbon_sources 72 19078 19079 Experimental_context 19110 19111 Temperature_shift 72 19078 19079 Experimental_context 19111 19112 Iron-limitation 72 19078 19079 Experimental_context 19112 19113 Reactive_oxygen_species 72 19078 19079 Experimental_context 19113 19114 Oxygen_limitation 72 19078 19079 Experimental_context 19114 19115 Drug_resistance 73 19079 19080 Material_source 19115 19116 fresh 73 19079 19080 Material_source 19116 19117 frozen 74 19080 19081 Fractionate 19117 19118 none 74 19080 19081 Fractionate 19118 19119 cell_compartment-cytosol 74 19080 19081 Fractionate 19119 19120 cell_compartment-nucleus 74 19080 19081 Fractionate 19120 19121 cell_compartment-mitochondria 74 19080 19081 Fractionate 19121 19122 cell_compartment-membrane 74 19080 19081 Fractionate 19122 19123 culture_supernatant 75 19081 19082 Purification 19123 19124 none 75 19081 19082 Purification 19124 19125 phenole_precipitation 75 19081 19082 Purification 19125 19126 purification_kit 75 19081 19082 Purification 19126 19127 TCA-precipitation 76 19082 19083 Lysis_buffer 19127 19128 Lysis_buffer1_(CHAPS/Urea) 76 19082 19083 Lysis_buffer 19128 19129 Lysis_buffer2_(CHAPS/Thiourea) 76 19082 19083 Lysis_buffer 19129 19130 Lysis_buffer3_(CHAPS/SB3-10/Thiourea) 76 19082 19083 Lysis_buffer 19130 19131 Lysis_buffer3_for_DIGE 76 19082 19083 Lysis_buffer 19131 19132 Lysis_buffer4_(ASB-14/Thiourea) 76 19082 19083 Lysis_buffer 19132 19133 Lysis_buffer5_(ASB-14/CHAPS_SB3-10/Thiourea) 76 19082 19083 Lysis_buffer 19133 19134 Lysis_buffer6_(C7_BzO) 76 19082 19083 Lysis_buffer 19134 19135 Lysis_buffer7_(CHAPS/C7_BzO) 77 19083 19084 Culture 19135 19136 liquid 77 19083 19084 Culture 19136 19137 solid 77 19083 19084 Culture 19137 19138 glycerol_stock 78 19084 19085 Life_cycle_status 19138 19139 mycelia 78 19084 19085 Life_cycle_status 19139 19140 conidia 78 19084 19085 Life_cycle_status 19140 19141 mixed 79 19085 19086 Temperature_(enter_NA_if_unknown) 0 0 [degC] 80 19086 19087 pH_(enter_NA_if_unknown) 0 0 [] 81 19087 19088 Medium 19141 19142 AMM 81 19087 19088 Medium 19142 19143 CM 81 19087 19088 Medium 19143 19144 YEPD_complete_medium 81 19087 19088 Medium 19144 19145 RPMI 82 19088 19089 Incubation_period_(enter_NA_if_unknown) 0 0 [min] 83 19089 19090 Glucose 0 0 [%] 84 19090 19091 Galactose 0 0 [%] 85 19091 19092 Ethanol 0 0 [%] 86 19092 19093 Glycerol 0 0 [%] 87 19093 19094 Sucrose 0 0 [%] 88 19094 19095 Lactate 0 0 [%] 89 19095 19096 Culture 19145 19146 liquid 89 19095 19096 Culture 19146 19147 solid 89 19095 19096 Culture 19147 19148 glycerol_stock 90 19096 19097 Life_cycle_status 19148 19149 mycelia 90 19096 19097 Life_cycle_status 19149 19150 conidia 90 19096 19097 Life_cycle_status 19150 19151 mixed 91 19097 19098 Temperature_(enter_NA_if_unknown) 0 0 [degC] 92 19098 19099 pH_(enter_NA_if_unknown) 0 0 [] 93 19099 19100 Medium 19151 19152 AMM 93 19099 19100 Medium 19152 19153 CM 93 19099 19100 Medium 19153 19154 YEPD_complete_medium 93 19099 19100 Medium 19154 19155 RPMI 94 19100 19101 Glucose 0 0 [%] 95 19101 19102 Galactose 0 0 [%] 96 19102 19103 Ethanol 0 0 [%] 97 19103 19104 Glycerol 0 0 [%] 98 19104 19105 Sucrose 0 0 [%] 99 19105 19106 Lactate 0 0 [%] 100 19106 19107 Name 19155 19156 H2O2 100 19106 19107 Name 19156 19157 diamide 100 19106 19107 Name 19157 -1 farnesol 101 19107 19108 Concentration 0 0 [mM] 102 19108 19109 Incubation_period_with_additive_(enter_NA_if_not_applicable) 0 0 [min] 103 19109 19110 Incubation_period_after_temperature_shift_(enter_NA_if_not_applicable) 0 0 [min] 104 19110 19111 Incubation_period_after_pH_shift_(enter_NA_if_not_applicable) 0 0 [min] 105 19111 19112 Incubation_period_after_medium_shift_(enter_NA_if_not_applicable) 0 0 [min] 106 19112 -1 Incubation_period_after_C_source_shift_(enter_NA_if_not_applicable) 0 0 [min]