Dear all,
After having read the general hints on the below mentioned page, I would like to comment a few things about external controls called LORECs. LORECs (libraries of random external controls) were thought to be a systems which may be adapted to the needs of a specific microarray setup in terms of GC-content and probe length. LOREC 1.0 which you are probably working with has a GC of about 50% and lengts of >500bp. If you want to work with them you may find it helpful to read Anja Koschmieders Diplomarbeit (in my old lab with Jorge or in Jörgs office). LORECs are not principally bad for Epoxislides (because its simply DNA), but for the spotting plate they were dissolved in spotting solution suitable for Aminoslides and also do not have aminolinkers. This may have caused the improper spot size. It may be a good idea to make them anew from clones, which is not much work. RNA-Pools should be still there, since we made plenty of it.

The whole system has not been published so far, although the proof-of-principle is well documented in the diploma thesis. But a broad field test was finally missing, because Anja could not do this and I also left. Thus if you are using the system I will be happy if you contact me, since I am still interested in publishing the LOREC principle. If you find this suitable for your work, I also may have ressources to continue this work with students here.

By end of the year I expect to have the Roche Lightcycler 480 system in my lab (RT-PCR in array format). Thus if you are interested in collaborations you are welcome.

Please use

for emails.

Cheers Marcus