oxidative_stress(0510152030) (MCE 5)
condition-dependent annotations:
control
1053 -
temporary_additive
1121 - H2O2
1122 - NaCl
1135 - calcium
1136 - isoamylalcohol
1145 - sorbitol
1146 - ammonium_sulfate
1148 - glucose
1123 - none
1055 -
incubation_period
min
condition 1
1053 -
temporary_additive
1121 - H2O2
1122 - NaCl
1135 - calcium
1136 - isoamylalcohol
1145 - sorbitol
1146 - ammonium_sulfate
1148 - glucose
1123 - none
1055 -
incubation_period
min
condition 2
1053 -
temporary_additive
1121 - H2O2
1122 - NaCl
1135 - calcium
1136 - isoamylalcohol
1145 - sorbitol
1146 - ammonium_sulfate
1148 - glucose
1123 - none
1055 -
incubation_period
min
condition 3
1053 -
temporary_additive
1121 - H2O2
1122 - NaCl
1135 - calcium
1136 - isoamylalcohol
1145 - sorbitol
1146 - ammonium_sulfate
1148 - glucose
1123 - none
1055 -
incubation_period
min
condition 4
1053 -
temporary_additive
1121 - H2O2
1122 - NaCl
1135 - calcium
1136 - isoamylalcohol
1145 - sorbitol
1146 - ammonium_sulfate
1148 - glucose
1123 - none
1055 -
incubation_period
min
condition 5
1053 -
temporary_additive
1121 - H2O2
1122 - NaCl
1135 - calcium
1136 - isoamylalcohol
1145 - sorbitol
1146 - ammonium_sulfate
1148 - glucose
1123 - none
1055 -
incubation_period
min
measurement-dependent annotations:
control
measurement 1 #48
2 -
array_series
3 -
array_individual
7 -
array_hybridisation
16 -
label_incorporation_rate
%
17 -
total_activity
cpm
39 -
experimentator
218 - aign
104 - bastuk
115 - becerra
105 - bier
88 - boer
190 - diehl
196 - fischer
191 - grahlmann
219 - grees
87 - hauser
91 - kornacker
112 - lagorce
107 - lombardia
90 - lutz
108 - meijer
92 - mendel
198 - reile
195 - scheideler
197 - schrenk
111 - steiner
110 - sueltmann
106 - tournu
89 - wilmer
93 - wittig
116 - yin
measurement 2 #49
2 -
array_series
3 -
array_individual
7 -
array_hybridisation
16 -
label_incorporation_rate
%
17 -
total_activity
cpm
39 -
experimentator
218 - aign
104 - bastuk
115 - becerra
105 - bier
88 - boer
190 - diehl
196 - fischer
191 - grahlmann
219 - grees
87 - hauser
91 - kornacker
112 - lagorce
107 - lombardia
90 - lutz
108 - meijer
92 - mendel
198 - reile
195 - scheideler
197 - schrenk
111 - steiner
110 - sueltmann
106 - tournu
89 - wilmer
93 - wittig
116 - yin
condition 1
measurement 1 #50
2 -
array_series
3 -
array_individual
7 -
array_hybridisation
16 -
label_incorporation_rate
%
17 -
total_activity
cpm
39 -
experimentator
218 - aign
104 - bastuk
115 - becerra
105 - bier
88 - boer
190 - diehl
196 - fischer
191 - grahlmann
219 - grees
87 - hauser
91 - kornacker
112 - lagorce
107 - lombardia
90 - lutz
108 - meijer
92 - mendel
198 - reile
195 - scheideler
197 - schrenk
111 - steiner
110 - sueltmann
106 - tournu
89 - wilmer
93 - wittig
116 - yin
measurement 2 #51
2 -
array_series
3 -
array_individual
7 -
array_hybridisation
16 -
label_incorporation_rate
%
17 -
total_activity
cpm
39 -
experimentator
218 - aign
104 - bastuk
115 - becerra
105 - bier
88 - boer
190 - diehl
196 - fischer
191 - grahlmann
219 - grees
87 - hauser
91 - kornacker
112 - lagorce
107 - lombardia
90 - lutz
108 - meijer
92 - mendel
198 - reile
195 - scheideler
197 - schrenk
111 - steiner
110 - sueltmann
106 - tournu
89 - wilmer
93 - wittig
116 - yin
condition 2
measurement 1 #52
2 -
array_series
3 -
array_individual
7 -
array_hybridisation
16 -
label_incorporation_rate
%
17 -
total_activity
cpm
39 -
experimentator
218 - aign
104 - bastuk
115 - becerra
105 - bier
88 - boer
190 - diehl
196 - fischer
191 - grahlmann
219 - grees
87 - hauser
91 - kornacker
112 - lagorce
107 - lombardia
90 - lutz
108 - meijer
92 - mendel
198 - reile
195 - scheideler
197 - schrenk
111 - steiner
110 - sueltmann
106 - tournu
89 - wilmer
93 - wittig
116 - yin
measurement 2 #53
2 -
array_series
3 -
array_individual
7 -
array_hybridisation
16 -
label_incorporation_rate
%
17 -
total_activity
cpm
39 -
experimentator
218 - aign
104 - bastuk
115 - becerra
105 - bier
88 - boer
190 - diehl
196 - fischer
191 - grahlmann
219 - grees
87 - hauser
91 - kornacker
112 - lagorce
107 - lombardia
90 - lutz
108 - meijer
92 - mendel
198 - reile
195 - scheideler
197 - schrenk
111 - steiner
110 - sueltmann
106 - tournu
89 - wilmer
93 - wittig
116 - yin
condition 3
measurement 1 #54
2 -
array_series
3 -
array_individual
7 -
array_hybridisation
16 -
label_incorporation_rate
%
17 -
total_activity
cpm
39 -
experimentator
218 - aign
104 - bastuk
115 - becerra
105 - bier
88 - boer
190 - diehl
196 - fischer
191 - grahlmann
219 - grees
87 - hauser
91 - kornacker
112 - lagorce
107 - lombardia
90 - lutz
108 - meijer
92 - mendel
198 - reile
195 - scheideler
197 - schrenk
111 - steiner
110 - sueltmann
106 - tournu
89 - wilmer
93 - wittig
116 - yin
measurement 2 #55
2 -
array_series
3 -
array_individual
7 -
array_hybridisation
16 -
label_incorporation_rate
%
17 -
total_activity
cpm
39 -
experimentator
218 - aign
104 - bastuk
115 - becerra
105 - bier
88 - boer
190 - diehl
196 - fischer
191 - grahlmann
219 - grees
87 - hauser
91 - kornacker
112 - lagorce
107 - lombardia
90 - lutz
108 - meijer
92 - mendel
198 - reile
195 - scheideler
197 - schrenk
111 - steiner
110 - sueltmann
106 - tournu
89 - wilmer
93 - wittig
116 - yin
condition 4
measurement 1 #56
2 -
array_series
3 -
array_individual
7 -
array_hybridisation
16 -
label_incorporation_rate
%
17 -
total_activity
cpm
39 -
experimentator
218 - aign
104 - bastuk
115 - becerra
105 - bier
88 - boer
190 - diehl
196 - fischer
191 - grahlmann
219 - grees
87 - hauser
91 - kornacker
112 - lagorce
107 - lombardia
90 - lutz
108 - meijer
92 - mendel
198 - reile
195 - scheideler
197 - schrenk
111 - steiner
110 - sueltmann
106 - tournu
89 - wilmer
93 - wittig
116 - yin
measurement 2 #57
2 -
array_series
3 -
array_individual
7 -
array_hybridisation
16 -
label_incorporation_rate
%
17 -
total_activity
cpm
39 -
experimentator
218 - aign
104 - bastuk
115 - becerra
105 - bier
88 - boer
190 - diehl
196 - fischer
191 - grahlmann
219 - grees
87 - hauser
91 - kornacker
112 - lagorce
107 - lombardia
90 - lutz
108 - meijer
92 - mendel
198 - reile
195 - scheideler
197 - schrenk
111 - steiner
110 - sueltmann
106 - tournu
89 - wilmer
93 - wittig
116 - yin
condition 5
measurement 1 #58
2 -
array_series
3 -
array_individual
7 -
array_hybridisation
16 -
label_incorporation_rate
%
17 -
total_activity
cpm
39 -
experimentator
218 - aign
104 - bastuk
115 - becerra
105 - bier
88 - boer
190 - diehl
196 - fischer
191 - grahlmann
219 - grees
87 - hauser
91 - kornacker
112 - lagorce
107 - lombardia
90 - lutz
108 - meijer
92 - mendel
198 - reile
195 - scheideler
197 - schrenk
111 - steiner
110 - sueltmann
106 - tournu
89 - wilmer
93 - wittig
116 - yin
measurement 2 #59
2 -
array_series
3 -
array_individual
7 -
array_hybridisation
16 -
label_incorporation_rate
%
17 -
total_activity
cpm
39 -
experimentator
218 - aign
104 - bastuk
115 - becerra
105 - bier
88 - boer
190 - diehl
196 - fischer
191 - grahlmann
219 - grees
87 - hauser
91 - kornacker
112 - lagorce
107 - lombardia
90 - lutz
108 - meijer
92 - mendel
198 - reile
195 - scheideler
197 - schrenk
111 - steiner
110 - sueltmann
106 - tournu
89 - wilmer
93 - wittig
116 - yin
measurement 3 #60
2 -
array_series
3 -
array_individual
7 -
array_hybridisation
16 -
label_incorporation_rate
%
17 -
total_activity
cpm
39 -
experimentator
218 - aign
104 - bastuk
115 - becerra
105 - bier
88 - boer
190 - diehl
196 - fischer
191 - grahlmann
219 - grees
87 - hauser
91 - kornacker
112 - lagorce
107 - lombardia
90 - lutz
108 - meijer
92 - mendel
198 - reile
195 - scheideler
197 - schrenk
111 - steiner
110 - sueltmann
106 - tournu
89 - wilmer
93 - wittig
116 - yin
measurement 4 #61
2 -
array_series
3 -
array_individual
7 -
array_hybridisation
16 -
label_incorporation_rate
%
17 -
total_activity
cpm
39 -
experimentator
218 - aign
104 - bastuk
115 - becerra
105 - bier
88 - boer
190 - diehl
196 - fischer
191 - grahlmann
219 - grees
87 - hauser
91 - kornacker
112 - lagorce
107 - lombardia
90 - lutz
108 - meijer
92 - mendel
198 - reile
195 - scheideler
197 - schrenk
111 - steiner
110 - sueltmann
106 - tournu
89 - wilmer
93 - wittig
116 - yin
constant annotations (unchanged throughout the MCE):
common_annotations
array
1 -
array_source
10 - self_made
11 - genome_systems
12 - clontech
13 - research_genetics
2 -
array_series
*** measurement - dependent ***
3 -
array_individual
*** measurement - dependent ***
4 -
array_support
14 - nylon
15 - polypropylene
16 - glass
67 -
array_support_width
mm
68 -
array_support_length
mm
41 -
array_surface_chemistry
117 - unknown_positive_charge
118 - poly_L_lysine
119 - aldehyde
120 - aminosilane
42 -
support_manufacturer
121 - Hybond
122 - Pall
123 - TeleChem
124 - Corning
125 - diehl
126 - stephan
43 -
Hybond_support_brand
127 - non_Hybond
128 - N+
44 -
Pall_support_brand
129 - non_Pall
130 - Biodyne_B
223 - polypropylene
45 -
Corning_support_brand
131 - non_Corning
132 - CMT_GAPS
46 -
TeleChem_support_brand
133 - non_TeleChem
134 - Aldehyde
135 - SuperAldehyde
5 -
spotted_material
17 - PCR
18 - colonies
19 - DNA-oligo
20 - PNA-oligo
69 -
spotted_material_property
200 - single_stranded
201 - double_stranded
70 -
number_of_elements_on_array_(without_duplicates)
71 -
elements_spotted_as
202 - single
203 - duplicate
204 - triplicate
47 -
spotted_material_purification
136 - non
137 - isopropanol_precipitation
138 - QIAquick
139 - Multiscreen_NU_Millipore
48 -
spotting_buffer
140 - 1.5M_NaCl_0.5M_NaOH
141 - 28mM_EDC_4.4volpromill_NMI
142 - spottingsolution_by_diehl
143 - MicroSpotting_solution_TeleChem
144 - 3xSSC
145 - 3xSSC_50%DMSO
227 - 150mM_sodiumphosphate_pH8.5
228 - 150mM_sodiumphosphate_pH8.5_0.01%SDS
49 -
spotting_robot
146 - SDDC-2_ESI
147 - Biogrid_Biorobotics
148 - Microgrid_II_Biorobotics
149 - Omnigrid_Genemachines
150 - QArray_Genetix
50 -
spotting_pins
151 - solid
152 - SMP-3_Telechem
153 - Biogrid_Biorobotics
51 -
relative_humidity
154 - defined
155 - estimated
156 - unknown(enter_0%)
52 -
relative_humidity
%
53 -
temperature
157 - defined
158 - estimated
159 - unknown(enter_0deg.C)
54 -
temperature
deg.C
55 -
postprocessing
160 - neutralizing_by_1.5MNaCL_0.5MTrispH7.5_1mMEDTA
161 - 2h_at_65deg.C
162 - succinic_anhydride_Stanford_protocol
163 - succinic_anhydride_protocol_by_diehl
164 - BSA_Tigr_protocol
165 - NaBH4_Telechem_protocol
77 -
uv_crosslink_applicable
224 - no(enter_0)
225 - yes
78 -
uv_crosslink_energy
x100microJpercm2
6 -
readfile
7 -
array_hybridisation
*** measurement - dependent ***
hybridisation
RNA_preparation
8 -
material_source
21 - fresh
22 - frozen
23 - RNA_later
94 - cDNA_subtraction
9 -
preparation_of_total_RNA
24 - trizol
25 - columns
26 - phenol
95 - not_applicable
10 -
preparation_of_PolyA+
27 - none
28 - dynabeads
29 - oligotex
30 - cellulose
96 - not_applicable
labeling
11 -
amount_of_RNA
microgram
40 -
amount_of_cDNA
microgram
12 -
enzym
31 - superscript
32 - AMV
33 - M-MLV
166 - OmniScript
97 - klenow
13 -
priming
34 - oligo_dT
35 - anchored_oligo_dT
36 - random_hexamer
37 - combined_oligo_dT-random_hexamer
98 - pcr_select
14 -
nucleotide
38 - dCTP
39 - dATP
167 - aa-dUTP
168 - dUTP
56 -
nucleotide_removal
169 - G50_Pharmacia
170 - ammium_acetate_isopropanol_precipitation
226 - Na_acetate_isopropanol_precipitation
171 - QIAquick_Qiagen
172 - GFX_columns_Pharmacia
173 - YM30_Amicon
15 -
label
40 - 33P
41 - 32P
42 - Cy3
43 - Cy5
174 - Alexa488
175 - Alexa594
66 -
days_from_calibration_date_applicable
193 - yes
194 - no(enter_0d)
38 -
days_from_calibration_date
d
16 -
label_incorporation_rate
*** measurement - dependent ***
17 -
total_activity
*** measurement - dependent ***
hybridisation_conditions
18 -
buffer
44 - 0.5M_Na_phosphate_pH7.2_7%_SDS
229 - 5x_SSC_0.1%_SDS
230 - Genisphere3DNA
231 - Genisphere3DNAformamide
232 - 3x_SSC_2.5x_denhardts_0.08%_SDS
45 - 5x_SSC_5x_denhardts_0.5%_SDS
46 - 4x_SSC_7.2%_Na_sarcosyl
47 - Clontech_Express_hyb
48 - ambion_Northern_max
221 - 3x_SSC_5x_denhardts_1%_SDS_5%_dextransulfate_50%_formamide
57 -
prehybridization_without_target
176 - no
177 - yes
19 -
competitors
49 - none
50 - tRNA
51 - Cot1
217 - dA20
52 - dA40
53 - salmon_sperm
99 - dA40_Cot1_salmonsperm
100 - dA40_Cot1_tRNA_salmonsperm
101 - dA40_Cot1_specificoligos_salmonsperm
102 - dA40_Cot1_specificoligos_tRNA_salmonsperm
75 -
hybridisation_device
211 - water_bath
212 - rotating_oven
76 -
hybridisation_instrument
213 - hybridisation_bottle
214 - hybridisation_chamber_corning
215 - hybridisation_chamber_TeleChem
216 - hybridisation_chamber_ClonTech
20 -
hybridisation_temp
deg.C
21 -
buffer_volume
ml
58 -
coverslip_used
178 - no_(enter_size_0)
179 - yes
59 -
coverslip_size
mmxmm
22 -
hybridisation_length
h
stringency_wash
23 -
wash_buffer
54 - 0.1xSSC_0.1%SDS
55 - 0.6xSSC_1%SDS
56 - 0.2xSSC_0.1%SDS
222 - 0.2xSSC
233 - 0.1xSSC
234 - 0.06xSSC
57 - 40mMNaPipH7_0.1%SDS
24 -
times
25 -
wash_length
min
26 -
temperature
deg.C
detection
60 -
exposure_time_applicable
180 - yes
181 - no_(enter_0h)
27 -
exposure_time
h
28 -
measuring_device
58 - MD_storm860_phos
59 - MD_storm860_red
60 - MD_storm860_blue
113 - MD_425
114 - MD_475A_phos
61 - Fuji_BAS1500
109 - Fuji_FLA3000
62 - BioRad
63 - ScanArray3000_GSI_Lumonics
182 - ScanArray5000_GSI_Lumonics
183 - Axon_4000a_Axon
72 -
scanning_software
205 - Fuji_Image_Reader_FLA3000_3000G_V1.0
206 - BAS_reader_V2.26beta
207 - Storm_scanner_control
208 - Typhoon_scanner_control_V1.0
209 - Scan_Array3000_V3.11_Controller_V2.03
210 - Scan Array_V2.1
73 -
spacial_resolution
microm
61 -
pixelsize
micromxmicrom
62 -
PMT_setting_applicable
184 - no(enter_0%)
185 - yes
63 -
PMT_setting
%
64 -
laser_power_setting_applicable
186 - no(enter_0%)
187 - yes
65 -
laser_power_setting
%
29 -
imaging_software
64 - AIS
65 - HDG
66 - XDotsReader
67 - imagene
68 - Xdigitise
188 - GenePixPro
189 - QuantArray
30 -
background_correction
69 - none
70 - local_background
71 - block_background
72 - empty_spots_average
73 - empty_spots_per_block
31 -
spot_detection
74 - all_spots
75 - only_positive_spots
74 -
spot_dimension_(diameter)
microm
32 -
spot_size
76 - fixed
77 - variable
33 -
intensity_measurement
78 - highest_value_in_spot_centre
79 - sum_of_pixels_within_boundary
80 - mean_of_pixels_within_boundary
sample
34 -
organism
81 - Saccharomyces_cerevisiae
82 - Arabidopsis_thaliana
83 - murine_biopsy
103 - murine_cell_line
84 - human_biopsy
85 - human_cell_line
86 - Bacillus_subtilis
192 - Trypanosoma_brucei
199 - Candida_albicans
220 - Neurospora_crassa
submission
35 -
date_of_entry_day
36 -
date_of_entry_month
37 -
date_of_entry_year
39 -
experimentator
*** measurement - dependent ***
organism_specific_annotations
genotype
1035 -
strain
1087 - FY1679
1088 - RS453
1089 - Y546
1090 - CEN_PK2
1091 - 3E2
1092 - 702
1124 - MYC730
1125 - T73
1126 - WC4alpha
1127 - YSH2alpha
1128 - FY73
1129 - L5528
1130 - L5743
1139 - W303
1140 - FY834
1141 - AR27
1142 - X2180
1143 - aGH1
1149 - SEY6210
1036 -
mating_type
1093 - a
1094 - alpha
1095 - both
transgenes_ordered_by_no_in_y1_genes
1037 -
genetic_variation
1100 - WT
1096 - mutation
1097 - ko
1098 - overexpression
1099 - inducible_promoter
1038 -
transgene
no_in_y1_genes
1056 -
2nd_genetic_variation
1100 - WT
1096 - mutation
1097 - ko
1098 - overexpression
1099 - inducible_promoter
1057 -
2nd_transgene
no_in_y1_genes
1058 -
3rd_genetic_variation
1100 - WT
1096 - mutation
1097 - ko
1098 - overexpression
1099 - inducible_promoter
1059 -
3rd_transgene
no_in_y1_genes
1060 -
4th_genetic_variation
1100 - WT
1096 - mutation
1097 - ko
1098 - overexpression
1099 - inducible_promoter
1061 -
4th_transgene
no_in_y1_genes
1062 -
5th_genetic_variation
1100 - WT
1096 - mutation
1097 - ko
1098 - overexpression
1099 - inducible_promoter
1063 -
5th_transgene
no_in_y1_genes
auxotrophic_marker
1039 -
LEU2
1101 - yes
1102 - no
1040 -
URA3
1103 - yes
1104 - no
1041 -
TRP1
1105 - yes
1106 - no
1042 -
HIS3
1107 - yes
1108 - no
1043 -
LYS2
1109 - yes
1110 - no
1044 -
ADE2
1111 - yes
1112 - no
growth_conditions
1066 -
culture
1137 - liquid
1138 - solid
1045 -
growth_phase
1113 - exponential
1131 - pseudo_hyphal
1114 - stationary
1147 - arrested
1046 -
temperature
deg.C
1064 -
temperature_shift_incubation_period
min
1047 -
energy_metabolism
1115 - aerob
1116 - anaerob
medium
1048 -
base
1117 - YPD
1118 - SDC
1120 - MV
1132 - YNB
1133 - YNB_without_aa_without_am_sulfate
1134 - YNB_without_calcium
1144 - CM
C_source
1049 -
glucose
%
1050 -
galactose
%
1051 -
ethanol
%
1052 -
glycerol
%
1065 -
sucrose
%
1067 -
lactate
%
additive
1053 -
temporary_additive
*** condition - dependent ***
1054 -
concentration
mM
1055 -
incubation_period
*** condition - dependent ***