M-CHIPS home

Characteristics:
 
Correspondence Analysis
Planar embedding
Interpretation
 
Storing annotations without freetext
Why?
How?
To what end?


The signals for each gene are converted into numbers by some imaging software and the numbers are stored. Although these numbers take the vast majority of the storage space, they are simply-structured and therefore easy to store.

But for biological interpretation of these numbers it is necessary to have detailed information about the investigated samples and experimental procedures (protocols) involved.

These experiment annotations are stored as well, mainly for three reasons:

  • One likes to conserve these data over time. To still have it after the experimenter is not available any more, maybe leaving a labjournal that is not detailed enough or not readable at all :-).

  • Also, in order to put the data into a larger context, that is to compare with experiments done somewhere else. If one spins down yeast at 4 degrees or at room temperature, it might well make a difference in transcription patterns observed. But one will not be able to know why without access to these parameters.

  • Third, experiment description as well as sample biology are complex, resulting in too many experimental parameters to handle by visual inspection.
To give an example, arabidopsis got a list accounting for 149 variables. ...